PB-Neo-TetO-Cdk12_Nterm_FlagHa
(Plasmid #127179)

• Purpose: N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
• Depositing Lab: Phil Sharp
• Publication: Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. Epub 2018 Nov 28.

Backbone

• Vector backbone: PBNeo-TetO-Dest
• Backbone manufacturer: Albert Cheng (Jackson Labs)
• Backbone size w/o insert (bp): 10369
• Total vector size (bp): 13274
• Modifications to backbone: None
• Vector type: Mammalian Expression ; Piggybac Transposase Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12
• Alt name: Cdk12
• Species: M. musculus (mouse)
• Insert Size (bp): 4533
• GenBank ID: NM_001109626.1
• Entrez Gene: Cdk12 (a.k.a. 1810022J16Rik, Crk7, Crkrs, D11Ertd752e, Pksc)
• Promoter: TetO Promoter
• Tag / Fusion Protein:
  • Flag- HA- Tandem Epitopes (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: atccacgctgttttgacctc
• 3′ sequencing primer: agaccgaggagagggttagg

Resource Information

• Supplemental Documents:
  • PBNeo_TetO_Cdk12_isoform1NFH.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry