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pCR8/GW_TOPO_Cdk12_Nterm_FlagHa
(Plasmid #127177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 2817
  • Total vector size (bp) 7350
  • Modifications to backbone
    None
  • Vector type
    Entry vector with transgene for gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12
  • Alt name
    Cdk12
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4533
  • GenBank ID
    NM_001109626.1
  • Entrez Gene
    Cdk12 (a.k.a. 1810022J16Rik, Crk7, Crkrs, D11Ertd752e, Pksc)
  • Promoter None
  • Tag / Fusion Protein
    • Flag- HA- Tandem Epitopes (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8/GW_TOPO_Cdk12_Nterm_FlagHa was a gift from Phil Sharp (Addgene plasmid # 127177 ; http://n2t.net/addgene:127177 ; RRID:Addgene_127177)

  • For your References section:

    CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Dubbury SJ, Boutz PL, Sharp PA. Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. Epub 2018 Nov 28. 10.1038/s41586-018-0758-y PubMed 30487607
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