pAN1779
(Plasmid
#127165)
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PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCustom MoClo backbone
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Vector typeSynthetic Biology
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAN1779 was a gift from Hana El-Samad (Addgene plasmid # 127165 ; http://n2t.net/addgene:127165 ; RRID:Addgene_127165) -
For your References section:
Modular and tunable biological feedback control using a de novo protein switch. Ng AH, Nguyen TH, Gomez-Schiavon M, Dods G, Langan RA, Boyken SE, Samson JA, Waldburger LM, Dueber JE, Baker D, El-Samad H. Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1425-7. doi: 10.1038/s41586-019-1425-7. 10.1038/s41586-019-1425-7 PubMed 31341280
