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Addgene

pAN1232
(Plasmid #127164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom MoClo Backbone
  • Vector type
    Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAN1232 was a gift from Hana El-Samad (Addgene plasmid # 127164 ; http://n2t.net/addgene:127164 ; RRID:Addgene_127164)

  • For your References section:

    Modular and tunable biological feedback control using a de novo protein switch. Ng AH, Nguyen TH, Gomez-Schiavon M, Dods G, Langan RA, Boyken SE, Samson JA, Waldburger LM, Dueber JE, Baker D, El-Samad H. Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1425-7. doi: 10.1038/s41586-019-1425-7. 10.1038/s41586-019-1425-7 PubMed 31341280
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