pET-Sac-Abeta(MC1-42)
(Plasmid #127151)

• Purpose: Expresses N-terminal cysteine Abeta(1-42)
• Depositing Lab: James Nowick
• Publication: Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-1200. doi: 10.1021/acs.biochem.1c00105. Epub 2021 Apr 1.

Backbone

• Vector backbone: pET vector
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Abeta(MC1-42)
• Species: H. sapiens (human)
• Insert Size (bp): 159
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: Sac1 (not destroyed)
• 5′ sequencing primer: 5'- ATG TGC GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG GTG -3'
• 3′ sequencing primer: 5'- CAC AAC GCC ACC AAC CAT CAG ACC GAT GAT AGC ACC CTT GTT AGA ACC CAC -3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry