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pET-Sac-Abeta(MC1-42)
(Plasmid #127151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET vector
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Abeta(MC1-42)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    159
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site Sac1 (not destroyed)
  • 5′ sequencing primer 5'- ATG TGC GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG GTG -3'
  • 3′ sequencing primer 5'- CAC AAC GCC ACC AAC CAT CAG ACC GAT GAT AGC ACC CTT GTT AGA ACC CAC -3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Sac-Abeta(MC1-42) was a gift from James Nowick (Addgene plasmid # 127151 ; http://n2t.net/addgene:127151 ; RRID:Addgene_127151)

  • For your References section:

    Expression of N-Terminal Cysteine Abeta(42) and Conjugation to Generate Fluorescent and Biotinylated Abeta(42). Zhang S, Guaglianone G, Morris MA, Yoo S, Howitz WJ, Xing L, Zheng JG, Jusuf H, Huizar G, Lin J, Kreutzer AG, Nowick JS. Biochemistry. 2021 Apr 20;60(15):1191-1200. doi: 10.1021/acs.biochem.1c00105. Epub 2021 Apr 1. 10.1021/acs.biochem.1c00105 PubMed 33793198

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