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pLENTI_RPS10_K138R
(Plasmid #127136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti_C_Myc_DDK_IRES_Puro
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPS10
  • Species
    H. sapiens (human)
  • Mutation
    K138R
  • Entrez Gene
    RPS10 (a.k.a. DBA9, S10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is one additional mutation present in RPS10- K24R, but this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTI_RPS10_K138R was a gift from Thomas Tuschl (Addgene plasmid # 127136 ; http://n2t.net/addgene:127136 ; RRID:Addgene_127136)

  • For your References section:

    The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. Meyer C, Garzia A, Morozov P, Molina H, Tuschl T. Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24. 10.1016/j.molcel.2019.12.024 PubMed 31981475
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