TLCV2-LoxP
(Plasmid #127098)

• Purpose: LentiCRISPR v2 was modified into an all-in-one dox inducible system.
• Depositing Lab: Adam Karpf
• Publication: Karpf Lab plasmids (unpublished)

Backbone

• Vector backbone: lentiCRISPR v2
• Modifications to backbone: LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression. The LoxP sites allow the entire insert to be deleted upon addition of Cre recombinase.
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9-2A-eGFP
• Alt name: Cas9
• Alt name: enhanced GFP
• gRNA/shRNA sequence: na
• Species: H. sapiens (human)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • TLCV2-LoxP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry