RFP-(SIM)1
(Plasmid #127094)

• Purpose: Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.
• Depositing Lab: Michael Rosen
• Publication: Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. Epub 2016 Jun 30.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6325
• Modifications to backbone: His10-tev-cloning sites-tev-RK see attachment
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry - SUMO interacting motif (SIM) from PIASx
• Species: H. sapiens (human)
• Insert Size (bp): 840
• Entrez Gene: PIAS2 (a.k.a. ARIP3, DIP, MIZ1, PIASX, SIZ2, ZMIZ4)
• Promoter: unknown
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7: 5'-TAATACGACTCACTATAGGG-3'
• 3′ sequencing primer: T7-Term: 5'-GCTAGTTATTGCTCAGCGG-3'

Resource Information

• Supplemental Documents:
  • pHTTKR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry