pMS17
(Plasmid #127088)

• Purpose: Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.
• Depositing Lab: Maggie Smith
• Publication: Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87. doi: 10.1093/nar/gni086.

Backbone

• Vector backbone: pSET152
• Backbone size w/o insert (bp): 4460
• Total vector size (bp): 5894
• Vector type: Streptomyce integration vector derived from phiC31 int/attP

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: tcp830
• Alt name: Tetracycline inducible promoter
• Species: Synthetic
• Insert Size (bp): 69

Gene/Insert 2

• Gene/Insert name: TetRiS
• Alt name: Tetracycline repressor (TetR) adapted for use in Streptomyces
• Species: E. coli
• Insert Size (bp): 634

Gene/Insert 3

• Gene/Insert name: terminator mmrt
• Species: Streptomyces
• Insert Size (bp): 144

Gene/Insert 4

• Gene/Insert name: Terminator fdt
• Species: E. coli
• Insert Size (bp): 426

Resource Information

• Supplemental Documents:
  • pMS17 (modified).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For research using this plasmid, please acknowledge the depositing laboratory and associated citation.