pHCKan-yibDp-GFPuv Citations (4)

Originally described in: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.
Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. PubMed Journal

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Articles Citing pHCKan-yibDp-GFPuv

Articles
Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease. Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang R, Lynch MD. Biotechnol Bioeng. 2020 Sep;117(9):2852-2860. doi: 10.1002/bit.27444. Epub 2020 Jun 20. PubMed
Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli. Menacho-Melgar R, Hennigan JN, Lynch MD. Biotechniques. 2021 Nov;71(5):566-572. doi: 10.2144/btn-2021-0055. Epub 2021 Aug 25. PubMed
Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli. Menacho-Melgar R, Lynch MD. Bio Protoc. 2022 Jan 20;12(2):e4297. doi: 10.21769/BioProtoc.4297. eCollection 2022 Jan 20. PubMed
2-Stage microfermentations. Li S, Ye Z, Moreb EA, Menacho-Melgar R, Golovsky M, Lynch MD. Metab Eng Commun. 2024 Apr 8;18:e00233. doi: 10.1016/j.mec.2024.e00233. eCollection 2024 Jun. PubMed

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