pB6-Hipp11-DTA-Capecchi-rev
(Plasmid
#127054)
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Purpose(Empty Backbone) B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCustom
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byDTA negative selection cassette is from Philippe Soriano. p15A backbone is from Sen Wu, Capecchi laboratory.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
AscI and PacI cloning sites near reported Hipp11 locus (Hippenmeyer et al., 2010), a highly recombinogenic locus.
Genomic sequence in pHipp11 plasmids #52544 and #52545 might be from 129/SvJ mouse according to the paper cited above.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB6-Hipp11-DTA-Capecchi-rev was a gift from Mario Capecchi (Addgene plasmid # 127054 ; http://n2t.net/addgene:127054 ; RRID:Addgene_127054)
