pJUMP18-pheS_NOC
(Plasmid
#127031)
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PurposeBasic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJUMP18-Uac
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Backbone manufacturerBackbone derived from SEVA.
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePart PheS_NOC
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SpeciesSynthetic
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MutationpheS A294G promiscuous mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Part based on sequence from "Marionette strains" publication (PMID:30478458 ) Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJUMP18-pheS_NOC was a gift from Chris French (Addgene plasmid # 127031 ; http://n2t.net/addgene:127031 ; RRID:Addgene_127031) -
For your References section:
Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology. Valenzuela-Ortega M, French C. Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 10.1093/synbio/ysab003. eCollection 2021. 10.1093/synbio/ysab003 PubMed 33623824