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pJUMP18-B0033-MV_RN
(Plasmid #127016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJUMP18-Uac
  • Backbone manufacturer
    Backbone derived from SEVA.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Part B0033m-MV_RN
  • Species
    Synthetic
  • Mutation
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RBS based on sequence from CIDAR MoClo toolkit (PMID: 26479688). Methionine-Valine translational start. Valine codon GUA following AUG inititiation codon has strong translation initiation in Escherichia coli (Looman et al; 1987. PMID: 3311730) Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJUMP18-B0033-MV_RN was a gift from Chris French (Addgene plasmid # 127016 ; http://n2t.net/addgene:127016 ; RRID:Addgene_127016)

  • For your References section:

    Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology. Valenzuela-Ortega M, French C. Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 10.1093/synbio/ysab003. eCollection 2021. 10.1093/synbio/ysab003 PubMed 33623824
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