pJUMP27ts[mCherry]-1A(lacZ)
(Plasmid
#126980)
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Purpose(Empty Backbone) Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJUMP27ts-1A
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Backbone manufacturerBackbone derived from SEVA.
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)JM109
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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MutationRepA A56V thermosensitive mutation
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJUMP27ts[mCherry]-1A(lacZ) was a gift from Chris French (Addgene plasmid # 126980 ; http://n2t.net/addgene:126980 ; RRID:Addgene_126980) -
For your References section:
Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology. Valenzuela-Ortega M, French C. Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 10.1093/synbio/ysab003. eCollection 2021. 10.1093/synbio/ysab003 PubMed 33623824
