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Addgene

pENTRY4_ Pi21
(Plasmid #126904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wheat_live_Cas9
  • gRNA/shRNA sequence
    AGATCGAGATCGTAAAACAA, ATATCAAAATCGTACCACAA, TATGGCACGAACTTGTATGT, TAGGGCATGAACTTGTATGT
  • Species
    Synthetic
  • Insert Size (bp)
    8726

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes gene specific sgRNAs driven by the wheat U6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTRY4_ Pi21 was a gift from Sophien Kamoun (Addgene plasmid # 126904 ; http://n2t.net/addgene:126904 ; RRID:Addgene_126904)
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