pICSL11085_GG_Wheat_live_Cas9_for
(Plasmid
#126881)
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PurposeEncodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepICH47742
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWheat_live_Cas9
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SpeciesSynthetic
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Insert Size (bp)8726
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICSL11085_GG_Wheat_live_Cas9_for was a gift from Sophien Kamoun (Addgene plasmid # 126881 ; http://n2t.net/addgene:126881 ; RRID:Addgene_126881)
