IDA3::NeonGreen exon2 (WS15)
(Plasmid
#126876)
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PurposeNeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHyg3
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Vector typeOther
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIDA3
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IDA3::NeonGreen exon2 (WS15) was a gift from Winfield Sale (Addgene plasmid # 126876 ; http://n2t.net/addgene:126876 ; RRID:Addgene_126876) -
For your References section:
The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS. Mol Biol Cell. 2018 Apr 15;29(8):886-896. doi: 10.1091/mbc.E17-12-0729. Epub 2018 Mar 29. 10.1091/mbc.E17-12-0729 PubMed 29467251