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Addgene

IDA3::NeonGreen N-term (WS14)
(Plasmid #126875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHyg3
  • Vector type
    Other
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IDA3
  • Tag / Fusion Protein
    • NeonGreen (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IDA3::NeonGreen N-term (WS14) was a gift from Winfield Sale (Addgene plasmid # 126875 ; http://n2t.net/addgene:126875 ; RRID:Addgene_126875)

  • For your References section:

    The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS. Mol Biol Cell. 2018 Apr 15;29(8):886-896. doi: 10.1091/mbc.E17-12-0729. Epub 2018 Mar 29. 10.1091/mbc.E17-12-0729 PubMed 29467251
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