pcDNA3.1 3xF-dyskerin
(Plasmid
#126870)
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PurposeExpresses 3xFlag dsykerin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDyskerin
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Alt nameNOLA4
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Alt nameDKC
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- 3xFlag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 3xF-dyskerin was a gift from Kathleen Collins (Addgene plasmid # 126870 ; http://n2t.net/addgene:126870 ; RRID:Addgene_126870) -
For your References section:
Specificity and stoichiometry of subunit interactions in the human telomerase holoenzyme assembled in vivo. Egan ED, Collins K. Mol Cell Biol. 2010 Jun;30(11):2775-86. doi: 10.1128/MCB.00151-10. Epub 2010 Mar 29. 10.1128/MCB.00151-10 PubMed 20351177