pET-21a(+) SurE D9A
(Plasmid
#12686)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-21a(+)
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSurE
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Insert Size (bp)741
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Mutationchanged Aspartate 9 to Alanine
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: The SurE insert contains an additional mutation (Q12R) that the depositor has confirmed does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-21a(+) SurE D9A was a gift from SeWon Suh (Addgene plasmid # 12686 ; http://n2t.net/addgene:12686 ; RRID:Addgene_12686) -
For your References section:
Crystal structure and functional analysis of the SurE protein identify a novel phosphatase family. Lee JY, Kwak JE, Moon J, Eom SH, Liong EC, Pedelacq JD, Berendzen J, Suh SW. Nat Struct Biol. 2001 Sep . 8(9):789-94. 10.1038/nsb0901-789 PubMed 11524683