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Pk_P12-Cd4-bio-His
(Plasmid #126845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6688
  • Total vector size (bp) 7587
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P12
  • Alt name
    PKNH_1137300
  • Alt name
    PKH_113620
  • Species
    Plasmodium knowlesi
  • Insert Size (bp)
    899
  • GenBank ID
    CAQ40949.1
  • Entrez Gene
    PKH_113620 (a.k.a. PKH_113620)
  • Promoter CMV
  • Tag / Fusion Protein
    • ratCd4(d3+4)-bio-6His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Insert was chemically synthesised.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pk_P12-Cd4-bio-His was a gift from Gavin Wright (Addgene plasmid # 126845 ; http://n2t.net/addgene:126845 ; RRID:Addgene_126845)

  • For your References section:

    A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance. Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, Ngasala B, Singh B, Farnert A, Wright GJ. Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5. 10.1186/s12936-020-3111-5 PubMed 31952523
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