Pk_CyRPA-Cd4-bio-His
(Plasmid #126841)

• Purpose: Expression of recombinant P. knowlesi protein in mammalian cells
• Depositing Lab: Gavin Wright
• Publication: Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6688
• Total vector size (bp): 7719
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CyRPA
• Alt name: PKNH_0515800
• Alt name: PKH_052740
• Species: Plasmodium knowlesi
• Insert Size (bp): 1031
• GenBank ID: CAQ38826.1
• Entrez Gene: PKH_052740 (a.k.a. PKH_052740)
• Promoter: CMV
• Tag / Fusion Protein:
  • ratCd4(d3+4)-bio-6His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • P4262.gb
• A portion of this plasmid was derived from a plasmid made by: Insert was chemically synthesised.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry