Skip to main content
Addgene

pET-21a(+)-LpxA
(Plasmid #12684)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UDP-N-acetylglucosamine acyltransferase
  • Alt name
    LpxA
  • Species
    Helicobacter pylori
  • Insert Size (bp)
    810
  • GenBank ID
    AE000511
  • Entrez Gene
    HP1375
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a(+)-LpxA was a gift from SeWon Suh (Addgene plasmid # 12684 ; http://n2t.net/addgene:12684 ; RRID:Addgene_12684)

  • For your References section:

    Crystal structure of UDP-N-acetylglucosamine acyltransferase from Helicobacter pylori. Lee BI, Suh SW. Proteins. 2003 Nov 15. 53(3):772-4. 10.1002/prot.10436 PubMed 14579368
Related items:
Commonly requested with: