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Addgene

pET-21a(+)-AroQ
(Plasmid #12682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    type II dehydroquinase
  • Alt name
    3-dehydroquinate dehydratase
  • Species
    Helicobacter pylori
  • Insert Size (bp)
    504
  • GenBank ID
    HP1038
  • Entrez Gene
    HP1038
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a(+)-AroQ was a gift from SeWon Suh (Addgene plasmid # 12682 ; http://n2t.net/addgene:12682 ; RRID:Addgene_12682)

  • For your References section:

    Crystal structure of the type II 3-dehydroquinase from Helicobacter pylori. Lee BI, Kwak JE, Suh SW. Proteins. 2003 Jun 1. 51(4):616-7. 10.1002/prot.10415 PubMed 12784220
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