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Addgene

pCfB4613
(Plasmid #126752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCfB258
  • Backbone manufacturer
    DOI: 10.1111/1567-1364.12118
  • Vector type
    Yeast Expression ; integrative plasmid
  • Selectable markers
    SpHiS5

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDC14
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    CDC14 (a.k.a. YFR028C, OAF3)
  • Promoter pTRX2_5xUAS

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGTGCAGGU AAAACA ATGCGTAGGAGTGTATACC
  • 3′ sequencing primer CGTGCGAU TTATTTCTTGATGGAGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB4613 was a gift from Michael Jensen (Addgene plasmid # 126752 ; http://n2t.net/addgene:126752 ; RRID:Addgene_126752)

  • For your References section:

    Engineering an NADPH/NADP(+) Redox Biosensor in Yeast. Zhang J, Sonnenschein N, Pihl TP, Pedersen KR, Jensen MK, Keasling JD. ACS Synth Biol. 2016 Dec 16;5(12):1546-1556. doi: 10.1021/acssynbio.6b00135. Epub 2016 Jul 25. 10.1021/acssynbio.6b00135 PubMed 27419466
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