pCfB4170
(Plasmid
#126750)
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PurposeExpresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS413
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyEGFP
- Promoter pTRX2_5xUAS
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ATCTGTCAU AAAACA ATGTCTAAAGGTGAAGAA
- 3′ sequencing primer CACGCGAU TTATTTGTACAATTCATCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCfB4170 was a gift from Michael Jensen (Addgene plasmid # 126750 ; http://n2t.net/addgene:126750 ; RRID:Addgene_126750) -
For your References section:
Engineering an NADPH/NADP(+) Redox Biosensor in Yeast. Zhang J, Sonnenschein N, Pihl TP, Pedersen KR, Jensen MK, Keasling JD. ACS Synth Biol. 2016 Dec 16;5(12):1546-1556. doi: 10.1021/acssynbio.6b00135. Epub 2016 Jul 25. 10.1021/acssynbio.6b00135 PubMed 27419466