pAG_P-HXT1-FAS1up
(Plasmid
#126747)
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Purposevector for replacing the promoter of FAS1 coding gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG 32
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Backbone manufacturerWaterham HR,et,al.
- Backbone size w/o insert (bp) 4160
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefas1 upstream
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SpeciesKomagataella phaffii
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Insert Size (bp)1200
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GenBank IDXM_002489597.1
- Promoter HXT1
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG_P-HXT1-FAS1up was a gift from Menghao Cai (Addgene plasmid # 126747 ; http://n2t.net/addgene:126747 ; RRID:Addgene_126747) -
For your References section:
Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast. Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M. Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. Epub 2019 May 9. 10.1016/j.ymben.2019.05.001 PubMed 31077813