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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
(Plasmid #126739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPIC 3.5K(DHis4)
  • Backbone manufacturer
    Liu Yiqi
  • Backbone size w/o insert (bp) 6469
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    adh2, acs1*, ald6
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4725
  • Mutation
    T3542C and A30S, S629N (please see depositor comments below)
  • GenBank ID
    CP020135.1, CP020138.1 CP020123.1
  • Promoter GAP
  • Tag / Fusion Protein
    • 6xHis,Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms the following mutations A30S and S629N do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* was a gift from Menghao Cai (Addgene plasmid # 126739 ; http://n2t.net/addgene:126739 ; RRID:Addgene_126739)

  • For your References section:

    Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast. Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M. Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. Epub 2019 May 9. 10.1016/j.ymben.2019.05.001 PubMed 31077813
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