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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
(Plasmid #126705)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126705 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PL451(ATCC)
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 7791
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    A. Victoria (Jellyfish)
  • Insert Size (bp)
    710

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR was a gift from Darrell Kotton (Addgene plasmid # 126705 ; http://n2t.net/addgene:126705 ; RRID:Addgene_126705)

  • For your References section:

    Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering. Kwong G, Marquez HA, Yang C, Wong JY, Kotton DN. Stem Cell Reports. 2019 Sep 10;13(3):499-514. doi: 10.1016/j.stemcr.2019.07.014. Epub 2019 Aug 15. 10.1016/j.stemcr.2019.07.014 PubMed 31422908
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