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Addgene

pHAGE2-EF1aL-mCherry-W
(Plasmid #126703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE2
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6977
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    705
  • Promoter Ef1aL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-EF1aL-mCherry-W was a gift from Darrell Kotton (Addgene plasmid # 126703 ; http://n2t.net/addgene:126703 ; RRID:Addgene_126703)

  • For your References section:

    Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. McCauley KB, Hawkins F, Serra M, Thomas DC, Jacob A, Kotton DN. Cell Stem Cell. 2017 Jun 1;20(6):844-857.e6. doi: 10.1016/j.stem.2017.03.001. Epub 2017 Mar 30. 10.1016/j.stem.2017.03.001 PubMed 28366587
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