pHAGE-UBC-hAAT-IRES-ZsGreen-W
(Plasmid #126702)

• Purpose: lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen
• Depositing Lab: Darrell Kotton
• Publication: Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39(2):133-41.

Backbone

• Vector backbone: pHAGE
• Backbone manufacturer: Derived in Kotton lab
• Backbone size w/o insert (bp): 6256
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen"
• Alt name: SERPINA1
• Alt name: a1AT
• Species: H. sapiens (human)
• Insert Size (bp): 2528
• Entrez Gene: SERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)
• Promoter: UBC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)

Resource Information

• Supplemental Documents:
  • pHAGE-UBC-hAAT-IRES-ZsGreen-W.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry