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Addgene

EF1a-TALEN_HD (SPC right)
(Plasmid #126695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF1a-TALEN
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6413
  • Vector type
    Mammalian Expression, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human SPC right
  • Insert Size (bp)
    1897
  • Mutation
    R451P in SPC
  • Promoter EF1
  • Tags / Fusion Proteins
    • NLS, truncated N term (N terminal on insert)
    • TAL, FokI (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence finds an R451P mutation in SPC and a deletion of the 0.5 repeat HD region when compared to the depositor's sequence. These differences do not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a-TALEN_HD (SPC right) was a gift from Darrell Kotton (Addgene plasmid # 126695 ; http://n2t.net/addgene:126695 ; RRID:Addgene_126695)

  • For your References section:

    Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells. Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers MF, Kotton DN. Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 10.1016/j.stem.2017.08.014. Epub 2017 Sep 28. 10.1016/j.stem.2017.08.014 PubMed 28965766
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