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Addgene

pHAGE-hSPC-TFEBeGFP-W
(Plasmid #126692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 10685
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TFEB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2187
  • Mutation
    Deleted Stop Codon on TFEB
  • Entrez Gene
    TFEB (a.k.a. ALPHATFEB, BHLHE35, TCFEB)
  • Promoter hSPC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CCTCTCCCTACGGACACAT
  • 3′ sequencing primer GGAGCAACATAGTTAAGAATACCAGTCAATCTTTC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Shawn Ferguson (Addgene plasmid # 38119)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP fused to 3'end of TFEB insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-hSPC-TFEBeGFP-W was a gift from Darrell Kotton (Addgene plasmid # 126692 ; http://n2t.net/addgene:126692 ; RRID:Addgene_126692)
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