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Addgene

pHAGE2-RSV-jGCaMP7f-W
(Plasmid #126691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE2
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6111
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP7f
  • Species
    R. norvegicus (rat), G. gallus (chicken); A. Victoria (Jellyfish)
  • Insert Size (bp)
    1353
  • Mutation
    H78K, A317L
  • Promoter RSV
  • Tag / Fusion Protein
    • 6xHIS, T7, Xpress (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GGTACAGTGCAGGGGAAAGAAT
  • 3′ sequencing primer CCACATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Douglas Kim & GENIE Project (Addgene plasmid # 104463)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-RSV-jGCaMP7f-W was a gift from Darrell Kotton (Addgene plasmid # 126691 ; http://n2t.net/addgene:126691 ; RRID:Addgene_126691)
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