pHAGE-hSPC-TagBFP-W
(Plasmid
#126689)
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PurposeContains Blue Fluorescent Protein insert and a lineage-specific human surfactant protein C (SPC) promoter driving BFP expression.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE
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Backbone manufacturerDerived in Kotton lab
- Backbone size w/o insert (bp) 9915
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTagBFP
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SpeciesSynthetic
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Insert Size (bp)763
- Promoter hSPC
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CCTCTCCCTACGGACACAT
- 3′ sequencing primer GGAAAGAATCAAGGAGGCCA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-hSPC-TagBFP-W was a gift from Darrell Kotton (Addgene plasmid # 126689 ; http://n2t.net/addgene:126689 ; RRID:Addgene_126689)
