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Addgene

pHAGE-EF1aL-eGFP-W
(Plasmid #126686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6978
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    A. Victoria (Jellyfish), Human promoter
  • Insert Size (bp)
    720
  • Promoter EF1aL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EF1aL-eGFP-W was a gift from Darrell Kotton (Addgene plasmid # 126686 ; http://n2t.net/addgene:126686 ; RRID:Addgene_126686)

  • For your References section:

    Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells. Jacob A, Vedaie M, Roberts DA, Thomas DC, Villacorta-Martin C, Alysandratos KD, Hawkins F, Kotton DN. Nat Protoc. 2019 Dec;14(12):3303-3332. doi: 10.1038/s41596-019-0220-0. Epub 2019 Nov 15. 10.1038/s41596-019-0220-0 PubMed 31732721
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