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Addgene

pET21a(+)-PA PdxB
(Plasmid #12668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Erythronate-4-phosphate dehydrogenase
  • Alt name
    pdxB
  • Insert Size (bp)
    1143
  • GenBank ID
    AE004091
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-PA PdxB was a gift from SeWon Suh (Addgene plasmid # 12668 ; http://n2t.net/addgene:12668 ; RRID:Addgene_12668)

  • For your References section:

    Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa. Ha JY, Lee JH, Kim KH, Kim DJ, Lee HH, Kim HK, Yoon HJ, Suh SW. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Feb 1. 62(Pt 2):139-41. 10.1107/S1744309106000649 PubMed 16511285
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