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Addgene

pCMVtight-RFP-IRES-CD
(Plasmid #126678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMVtight Puro
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 7900
  • Total vector size (bp) 9100
  • Modifications to backbone
    cloned out eGFP
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFP-IRES-CD
  • Alt name
    cytosine-deaminase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1200
  • Entrez Gene
    FCY1 (a.k.a. YPR062W)
  • Promoter tight TRE

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CD: Addgene #35102; pLenti CMVtight vector: Addgene #26431

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVtight-RFP-IRES-CD was a gift from Joan Massague (Addgene plasmid # 126678 ; http://n2t.net/addgene:126678 ; RRID:Addgene_126678)

  • For your References section:

    Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization. Basnet H, Tian L, Ganesh K, Huang YH, Macalinao DG, Brogi E, Finley LW, Massague J. Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627. 10.7554/eLife.43627 PubMed 30912515
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