pCMVtight-UPRT-T2A-RFP-IRES-CD
(Plasmid #126677)

• Purpose: Doxycycline inducible expression of UPRT and CD along with reporter RFP
• Depositing Lab: Joan Massague
• Publication: Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627.

Backbone

• Vector backbone: pLenti CMVtight Puro
• Backbone manufacturer: addgene
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 9900
• Modifications to backbone: cloned out eGFP
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UPRT-T2A-RFP-IRES-CD
• Alt name: uracil phosphoribosyltransferase
• Alt name: TGME49_312480
• Alt name: cytosine-deaminase
• Species: S. cerevisiae (budding yeast); Toxoplasma gondii
• Insert Size (bp): 2000
• GenBank ID: XP_002364470.1
• Entrez Gene: FCY1 (a.k.a. YPR062W)
• Promoter: tight TRE

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMVtightFORW
• 3′ sequencing primer: WPRErev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: UPRT: Addgene #47110; CD: Addgene #35102; pLenti CMVtight vector: Addgene #26431
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry