pET-22b(+)-UTPase
(Plasmid #12667)

• Depositing Lab: SeWon Suh
• Publication: Han et al Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1147-9.

Backbone

• Vector backbone: pET-22b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5493
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dUTP pyrophosphatase
• Alt name: DUT1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 444
• GenBank ID: NC_001134
• Entrez Gene: DUT1
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a H99R mutation in the insert. The depositing author is aware of the mutation, however the effects of the mutation on protein function are unknown.