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pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK
(Plasmid #126651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWS-TK6
  • Backbone manufacturer
    Sen Wu, Capecchi laboratory
  • Vector type
    Mouse Targeting, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Lrig1
  • Species
    M. musculus (mouse)
  • Mutation
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
  • Entrez Gene
    Lrig1 (a.k.a. D6Bwg0781e, Img, LIG-1)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon. FRT-Neo-FRT is from pFNF (#22687).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.

The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35) Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.

Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

Mice available from the MMRRC (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=69516)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK was a gift from Mario Capecchi (Addgene plasmid # 126651 ; http://n2t.net/addgene:126651 ; RRID:Addgene_126651)

  • For your References section:

    Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Nam HS, Capecchi MR. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. 10.1186/s13064-020-00139-5 PubMed 32183906
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