pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK
(Plasmid
#126651)
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PurposeB6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWS-TK6
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Backbone manufacturerSen Wu, Capecchi laboratory
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Vector typeMouse Targeting, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLrig1
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SpeciesM. musculus (mouse)
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MutationC-terminal fusion of T2A-sfGFP-iCre-ERT2
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Entrez GeneLrig1 (a.k.a. D6Bwg0781e, Img, LIG-1)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bysfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon. FRT-Neo-FRT is from pFNF (#22687).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35) Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Mice available from the MMRRC (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=69516)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK was a gift from Mario Capecchi (Addgene plasmid # 126651 ; http://n2t.net/addgene:126651 ; RRID:Addgene_126651) -
For your References section:
Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Nam HS, Capecchi MR. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. 10.1186/s13064-020-00139-5 PubMed 32183906