TrmD_HaeIn/pET28b(+) N,C-tag
(Plasmid
#12665)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET-28b(+)
-
Backbone manufacturerMerck
- Backbone size w/o insert (bp) 5369
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nametRNA (guanine-N(1)-)-methyltransferase
-
Alt nameTrmD
-
SpeciesHaemophilus influenzae
-
Insert Size (bp)741
-
GenBank IDL42023
-
Entrez GenetrmD
-
Tags
/ Fusion Proteins
- His (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TrmD_HaeIn/pET28b(+) N,C-tag was a gift from SeWon Suh (Addgene plasmid # 12665 ; http://n2t.net/addgene:12665 ; RRID:Addgene_12665) -
For your References section:
Crystallization and preliminary X-ray crystallographic analysis of tRNA(m1G37)methyltransferase from Haemophilus influenzae. Kim HW, Ahn HJ, Yoon HJ, Kim HW, Baek SH, Suh SW. Acta Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):183-4. 10.1107/S0907444902019716 PubMed 12499565
Map uploaded by the depositor.
