Skip to main content
Addgene

p5343_UC57
(Plasmid #126645)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 13545
  • Modifications to backbone
    None
  • Vector type
    Synthetic Biology
  • Selectable markers
    mercury chloride

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Environmental plasmid p5343
  • Alt name
    p5343_UC57,
  • Species
    NA
  • Insert Size (bp)
    8102
  • Promoter NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer TGAGCACCTTCGGCGACG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We isolated the native 8 kb plasmid p5343 from a groundwater sample and synthesized it

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5343_UC57 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 126645 ; http://n2t.net/addgene:126645 ; RRID:Addgene_126645)

  • For your References section:

    Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates. Kothari A, Soneja D, Tang A, Carlson HK, Deutschbauer AM, Mukhopadhyay A. mSystems. 2019 Dec 17;4(6). pii: 4/6/e00588-19. doi: 10.1128/mSystems.00588-19. 10.1128/mSystems.00588-19 PubMed 31848306
Related items: