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AsCas12a-DR-cr in PBCSK-
(Plasmid #126640)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126640 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBCSK-
  • Backbone size w/o insert (bp) 3659
  • Total vector size (bp) 3694
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCas12a Full Length Direct Repeat crRNA
  • Species
    Synthetic
  • Insert Size (bp)
    35
  • Promoter U6 Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer U6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AsCas12a-DR-cr in PBCSK- was a gift from Scot Wolfe (Addgene plasmid # 126640 ; http://n2t.net/addgene:126640 ; RRID:Addgene_126640)

  • For your References section:

    Enhanced Cas12a editing in mammalian cells and zebrafish. Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184. 10.1093/nar/gkz184 PubMed 30892626
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