AsCas12a-2C-NLS in pCSDest
(Plasmid
#126637)
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PurposeExpresses AsCas12a-2C-NLS in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSDest
- Backbone size w/o insert (bp) 4086
- Total vector size (bp) 8163
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAsCas12a-2C-NLS
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Alt nameAsCpf1-2C-NLS
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SpeciesSynthetic
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Insert Size (bp)4077
- Promoter CMV IE94 promoter
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Tag
/ Fusion Protein
- 3xHuman influenza hemagglutinin (HA)-Tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AsCas12a-2C-NLS in pCSDest was a gift from Scot Wolfe (Addgene plasmid # 126637 ; http://n2t.net/addgene:126637 ; RRID:Addgene_126637) -
For your References section:
Enhanced Cas12a editing in mammalian cells and zebrafish. Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184. 10.1093/nar/gkz184 PubMed 30892626
