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pcDNA3-LumiLuc
(Plasmid #126622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 5962
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LumiLuc
  • Species
    Synthetic
  • Insert Size (bp)
    516
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer ATTTAGGTGACACTATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-LumiLuc was a gift from Huiwang Ai (Addgene plasmid # 126622 ; http://n2t.net/addgene:126622 ; RRID:Addgene_126622)

  • For your References section:

    ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. Yeh HW, Xiong Y, Wu T, Chen M, Ji A, Li X, Ai HW. ACS Chem Biol. 2019 Apr 17. doi: 10.1021/acschembio.9b00150. 10.1021/acschembio.9b00150 PubMed 30969754
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