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Addgene

RCAS-Flag-BRAFV600E
(Plasmid #126615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS-Y
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRAF
  • Alt name
    B-Raf proto-oncogene serine/threonine-protein kinase
  • Species
    H. sapiens (human)
  • Mutation
    V600E
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A PacI-ClaI PCR fragment of Flag-tagged BRAFV600E from Addgene plasmid # 53156 was inserted into RCAS-Y.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS-Flag-BRAFV600E was a gift from Yi Li (Addgene plasmid # 126615 ; http://n2t.net/addgene:126615 ; RRID:Addgene_126615)

  • For your References section:

    Mammary Precancerous Stem and Non-Stem Cells Evolve into Cancers of Distinct Subtypes. Bu W, Liu Z, Jiang W, Nagi C, Huang S, Edwards DP, Jo E, Mo Q, Creighton CJ, Hilsenbeck SG, Leavitt AD, Lewis MT, Wong STC, Li Y. Cancer Res. 2019 Jan 1;79(1):61-71. doi: 10.1158/0008-5472.CAN-18-1087. Epub 2018 Nov 6. 10.1158/0008-5472.CAN-18-1087 PubMed 30401712
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