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pB-puro-mU6-CNPsgRNA
(Plasmid #126610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-puro
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CNP sgRNA
  • gRNA/shRNA sequence
    ATGTCATCCTCAGGAGCAA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cnp (a.k.a. CNPase, Cnp-1, Cnp1)
  • Promoter mouse U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer CAGCACAAAAGGAAACTCACCCTAACTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-puro-mU6-CNPsgRNA was a gift from Xiaozhong Wang (Addgene plasmid # 126610 ; http://n2t.net/addgene:126610 ; RRID:Addgene_126610)

  • For your References section:

    The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. Unlu I, Lu Y, Wang X. J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 10.1074/jbc.RA118.004872. Epub 2018 Oct 24. 10.1074/jbc.RA118.004872 PubMed 30355738
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