pB-bsd-Flag-Trl1
(Plasmid #126609)

• Purpose: Expresses N-terminal Flag-tagged yeast Trl1
• Depositing Lab: Xiaozhong Wang
• Publication: Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 10.1074/jbc.RA118.004872. Epub 2018 Oct 24.

Backbone

• Vector backbone: PB-bsd
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tRNA ligase 1
• Alt name: Trl1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2484
• Entrez Gene: TRL1 (a.k.a. YJL087C, LIG1, RLG1)
• Promoter: CAG
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAGCCTCTGCTAACCATGTTCATGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.