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Addgene

FLAG-SENP2 NPm
(Plasmid #126593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 6733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SENP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1596
  • Mutation
    Deletion of amino acids 1-65 and L329A/L331A in the NES
  • GenBank ID
    59343
  • Entrez Gene
    SENP2 (a.k.a. AXAM2, SMT3IP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (unknown if destroyed)
  • 3′ cloning site Eco RV (unknown if destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer bGH_R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deletion of N terminus to remove nuclear pore targetting sequence and mutation of two residues in the NES to reduce nuclear export. The NPm mutant is expressed in the nucleoplasm and partially in the cytoplasm.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-SENP2 NPm was a gift from Joanna Morris (Addgene plasmid # 126593 ; http://n2t.net/addgene:126593 ; RRID:Addgene_126593)

  • For your References section:

    The deSUMOylase SENP2 coordinates homologous recombination and nonhomologous end joining by independent mechanisms. Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley JF, Morris JR. Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. Epub 2019 Feb 22. 10.1101/gad.321125.118 PubMed 30796017
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